Part:BBa_M45698

pCstA+RBS+amilGFP used to indication of low glucose concentration

This BioBrick comsists of three parts: the promoter PcstA (BBa_K118011), the RBS (BBa_B0034) and the chromoprotein amilGFP. The increase of activity by adenylate cyclase under low glucose concentrations, results in cAMP binding to the cAMP receptor protein, and activating the promoter for downstream expression. This promoter has been ligated to an RBS as an intermediate step of ligations. Furthermore, The chromoprotein is from the coral Acropora millepora. It naturally exhibits strong yellow color when expressed. The color is readily visible to naked eye both in LB-culture and on agar plates. Color development can be seen in less than 24 hours of incubation.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]